| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075594 |
| Chromosome: | chromosome 16 |
| Location: | 5655640 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681000 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATCAGGAAGCGCGCACGCACACATCCACACGTCGGTTGACTTTTACTA |
| Internal bar code: | TGGCGACTACACGAACGCAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 10788 |
| LEAP-Seq percent confirming: | 55.814 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCAGTGGTGTCGTACAA |
| Suggested primer 2: | TTTGTACCGCTGGGCATGAT |