| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075597 |
| Chromosome: | chromosome 1 |
| Location: | 7755147 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g054600 | (1 of 8) IPR000157 - Toll/interleukin-1 receptor homology (TIR) domain | 3'UTR | |
| Cre01.g054650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACATGTCCTGTTTCCGCGTTGCTTCTTTCTACTTTCTGACACACGTAC |
| Internal bar code: | GGGGTGGACCGCCTGATTAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2205 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATTCCTGTAGGATGGGGC |
| Suggested primer 2: | ATTACCTCGCTCTTCCCCCT |