| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075605 |
| Chromosome: | plastome |
| Location: | 192904 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802334 | orf528 | unknown ORF | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGAGTTTACACATTTTAAAAAAGTGTGACATACTTCTTTTATTTTACT |
| Internal bar code: | TGAAGAATAGTGGTGTGCACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 380 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCTTCGTTGGAGCTTCT |
| Suggested primer 2: | CGCACGAGGCAGCATTAAAA |