Insertion junction: CLIP2.075609_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.075609
Chromosome:chromosome 9
Location:83927
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre09.g386250 SMM33 S-adenosyl-L-methionine-dependent methyltransferase; (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGTGCCACCAGGGCCTGCCGCGGGCTGACATCGTGTTCAGTAGGGACT
Internal bar code:AAGGCGAAAGTATGTCAGTGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:622
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCACCTCCACAATCACTC
Suggested primer 2:TAGCTCCCTACGTCAACGGA