| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.075611 |
| Chromosome: | chromosome 17 |
| Location: | 141472 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g696850 | POB16 | Proteome of basal body 16; (1 of 2) K17279 - receptor expression-enhancing protein 5/6 (REEP5_6) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCGGACCATAATGAGTACCACTGCTACAGCATATAAAACCCAACCCA |
| Internal bar code: | TATAACATAGAGTAACGACTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2228 |
| LEAP-Seq percent confirming: | 14.2857 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTTTTACCCAGGACGAC |
| Suggested primer 2: | TTGCTGTGGATGTGCAAAGC |