Insertion junction: CLIP2.075622_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.075622
Chromosome:chromosome 6
Location:2565358
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre06.g269601 (1 of 3) 2.4.1.19 - Cyclomaltodextrin glucanotransferase / Cyclodextrin glycosyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGACCTCGACGGCCACCGCAGCAGGCAGCGCGACCGCCTTGTTCTGCCC
Internal bar code:GTCATGCATCCCGTCGCACTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:311
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTCACTGTCAAACCGCACAC
Suggested primer 2:GTGTGCGGTTTGACAGTGAC