| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.075638 |
| Chromosome: | chromosome 12 |
| Location: | 9055209 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544650 | (1 of 10) PF13489 - Methyltransferase domain (Methyltransf_23) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGAACGCGTCCAGTGCGGCGTGATTGGGCGTGACCGCGTGAGCATAG |
| Internal bar code: | CCGCGTGGAGAATATTGGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1787 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACCTAGCGCTCCAGTAAG |
| Suggested primer 2: | ATTGCTATCGTACTCGCGCA |