| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.075649 |
| Chromosome: | chromosome 12 |
| Location: | 606067 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493600 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAATAAATGATGGTGATGTTGTCTTATCACGTTTCCTCGAGACGGTTGTG |
| Internal bar code: | GGTTTGCGCGGGACATTGATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2054 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCATCACTGTTTCCCCGG |
| Suggested primer 2: | CGCTTTACGCTGGACATTGG |