| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.075659 |
| Chromosome: | chromosome 10 |
| Location: | 4147335 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448800 | (1 of 1) PF09186 - Domain of unknown function (DUF1949) (DUF1949) | 3'UTR | |
| Cre10.g448850 | FAP135,ODA5AK,CAL5 | (1 of 3) 3.4.22.54 - Calpain-3 / Muscle-specific calcium-activated neutral protease 3; Calpain-Like Flagellar Associated Protein 135 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACAGCAAAATTCCAAACGCCTGCATCCATGTTGCCAGCCGCGTGCCGA |
| Internal bar code: | TGGTTAGTATTATTACTCAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3144 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACACAGACAGAGGCATGT |
| Suggested primer 2: | CATGCTCGCCTCGATAGTGT |