| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.075673 |
| Chromosome: | mating type plus |
| Location: | 353565 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g254200 | SRL1C,SRLc | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGTCTACCCGGCCCAGATGCTTACAGCCTATGCACAAATTCCAAAACG |
| Internal bar code: | GAATACTTGACCTTCGGGTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1177 |
| LEAP-Seq percent confirming: | 2.77778 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAACCGGTGACTCTCGTC |
| Suggested primer 2: | TTCCACGGTGACAGCTCTTC |