| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.075674 |
| Chromosome: | chromosome 13 |
| Location: | 2607004 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580900 | (1 of 1) K07178 - RIO kinase 1 (RIOK1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGGACAGTATAAATTTGAAGGGAGGATGTGGACTTGTCAACTTGCAT |
| Internal bar code: | CAGAGAGTCACAGTGTCGAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4729 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGAACTCCTCAGCTGCAA |
| Suggested primer 2: | GGCCAACACGCAAAGGATTT |