| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.075687 |
| Chromosome: | chromosome 10 |
| Location: | 286988 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419750 | RPB3 | DNA-directed RNA polymerase II, 36 kDa polypeptide; (1 of 1) K03011 - DNA-directed RNA polymerase II subunit RPB3 (RPB3, POLR2C) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCACAGTTATCAGTCTGCCCGCCAATTTTCGGCTCCTTGCGCTTCGGA |
| Internal bar code: | ACCTTCCGGAAAAGAATGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1643 |
| LEAP-Seq percent confirming: | 77.1429 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGTCACTGTGCACAAAAG |
| Suggested primer 2: | GGGCATTGAGGGAAGTGACA |