| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.075691 |
| Chromosome: | chromosome 7 |
| Location: | 4085012 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g340750 | EXN11 | RNaseT, SDN2 homolog; (1 of 1) PTHR12801:SF69 - RNA EXONUCLEASE NEF-SP-RELATED | 5'UTR |
| Cre07.g342950 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAATAGGCTTCATTTGTTCACGAGCTCAACCTCGTTGCACCCGCACGCT |
| Internal bar code: | GGTACCTTTACGATTTCGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3333 |
| LEAP-Seq percent confirming: | 64.2276 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 123 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTTTCCCGATACCCGTAG |
| Suggested primer 2: | GTAGGCGTTCTGTGTGTGGA |