| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.075695 |
| Chromosome: | chromosome 12 |
| Location: | 9662654 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557503 | (1 of 5) PF13676 - TIR domain (TIR_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGGGCAGCACACGTGGCAGCACATGGGGGAAATGGAGCGGGTACGGA |
| Internal bar code: | GAGTATACACTTACGTGCTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 74 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCAGGGAATGGAAATGGA |
| Suggested primer 2: | ATGAAGGGAGCACGCAAAGA |