| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.075714 |
| Chromosome: | chromosome 12 |
| Location: | 6150819 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535450 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACAGCTCTGTGGCATCGCCTGTGACTGCTCACCTCACTCGCTTACTTC |
| Internal bar code: | AGCGGGATCCGTCCGACCACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 891 |
| LEAP-Seq percent confirming: | 91.3043 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCCCTTGAAGAGCCTAA |
| Suggested primer 2: | CGGATCAAATTCCAGCAGCG |