| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075717 |
| Chromosome: | plastome |
| Location: | 74234 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802293 | orf202 | Wendy transposon | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAAGTAAGCCGTGGCACTGGCTTGAAAGTCCAAGGTGCATAACCTATT |
| Internal bar code: | CAGTCCGTAGATGGTAGCCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 459 |
| LEAP-Seq percent confirming: | 73.0769 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACCTCCCTTTCACGGAG |
| Suggested primer 2: | GCGGTGCAGCTTGTGTTTTA |