| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.075721 |
| Chromosome: | plastome |
| Location: | 193230 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802334 | orf528 | unknown ORF | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTAATTTTGTTGTCAGAGTTTGGGTTAAAGGGTTTTTGAAAATATATT |
| Internal bar code: | CGTTCCGCAAAGATAGGCGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 63 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTCAGTGGCTGTTGAAGT |
| Suggested primer 2: | TGTGGTTAAGCTCGAACCCC |