| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.075730 |
| Chromosome: | chromosome 12 |
| Location: | 4692108 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522550 | FLA8,KIN2-1 | Kinesin II Motor Protein; (1 of 2) K10394 - kinesin family member 3/17 (KIF3_17) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCATCTGGGCGCGGCGTGTGTTCGCTTCAGAACGTCCCACCAGATGCA |
| Internal bar code: | CTCATTACCGTAAGTCACGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4835 |
| LEAP-Seq percent confirming: | 94.6667 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTGGTTCGTGTCGGTCAG |
| Suggested primer 2: | CCGTTTGGTCGTTGTGTGTC |