| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075734 |
| Chromosome: | chromosome 3 |
| Location: | 5156570 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181000 | (1 of 6) 2.8.2.8 - [Heparan sulfate]-glucosamine N-sulfotransferase / PAPS:N-desulfoheparin sulfotransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCTGCCAGTGGGCCTGGGCTGAGCCTGCCTTGGGTTGAAGAACGCCAT |
| Internal bar code: | GGATTCGGGCATTTTGTGTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5398 |
| LEAP-Seq percent confirming: | 98.4127 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAATTGCACACACGTCAGT |
| Suggested primer 2: | AGAAACGTCCGCACTGATGT |