Insertion junction: CLIP2.075735_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.075735
Chromosome:chromosome 10
Location:3040817
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g440900 RRP43 Rrp42-like exosome component; (1 of 1) K12586 - exosome complex component RRP43 (RRP43, EXOSC8, OIP2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCACTTGACACGTTGCATACCACCAAGCCCACACAAACTAACAGACGTC
Internal bar code:ACTCAACTGAATACAGGATAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1607
LEAP-Seq percent confirming:95.2381
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCTCAGTGTGGAAATGGC
Suggested primer 2:CTGTGAGTGGTTGGGGGTAC