Insertion junction: CLIP2.075735_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.075735
Chromosome:chromosome 10
Location:3040856
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g440900 RRP43 Rrp42-like exosome component; (1 of 1) K12586 - exosome complex component RRP43 (RRP43, EXOSC8, OIP2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGACTTGCGTGACCAAGGAGTTTGAGGACAATTGAATGGGGCTCTGCCC
Internal bar code:ACTCAACTGAATACAGGATAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:739
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGAGTGGTTGGGGGTAC
Suggested primer 2:GTGCTCAGTGTGGAAATGGC