| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.075744 |
| Chromosome: | chromosome 12 |
| Location: | 4638210 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522150 | FAP194 | (1 of 239) IPR016024 - Armadillo-type fold; Flagellar Associated Protein 194 | 3'UTR |
| Cre12.g522200 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGAGTATCAGGTTGCTCCGAGACAAAAACACGCTGCGCTCCTCAGCTG |
| Internal bar code: | AGACTCCGATGAGCTGGTAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3524 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCGTGAGGGAACATGGT |
| Suggested primer 2: | TCTGTGGCGGTGGTAGTAGA |