| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.075744 |
| Chromosome: | chromosome 17 |
| Location: | 4569080 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g731800 | (1 of 2) PTHR10913 - FOLLISTATIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCATCTCCTCCATGACCAACAGCATGCCGTCTGCCCCACAGGCTAGTC |
| Internal bar code: | TATGAGCCCGCTTCGTAGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3466 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGCCATGTCGCTTGTTG |
| Suggested primer 2: | CCTTACCCATCCCTACCCCA |