| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075745 |
| Chromosome: | chromosome 8 |
| Location: | 1212686 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g800904 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTAACGAAATGCGGACTCGCCCAACATGTCGGCACGCCCGCACGCGCAC |
| Internal bar code: | TGCGTTTAAGATCGGGGGAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2187 |
| LEAP-Seq percent confirming: | 34.3434 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 99 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGTGAACCATTGGCGCAC |
| Suggested primer 2: | TTTGGTCTGCTCGGCTGTAG |