| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.075756 |
| Chromosome: | chromosome 14 |
| Location: | 144177 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g608452 | (1 of 1) K11366 - ubiquitin carboxyl-terminal hydrolase 22/27/51 [EC:3.4.19.12] (USP22_27_51, UBP8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGTCGGGTCGCGCCACGTATGCGCGAACCCACATGTGCCAGGGCTGC |
| Internal bar code: | TGTAGAGTGCGACTAAGAAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 190 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCAACCACAGATAAGCCT |
| Suggested primer 2: | ACGTCGGCACTTCACTTCAT |