Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.075757 |
Chromosome: | chromosome 12 |
Location: | 1666456 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g486551 | PFH19,PHX19 | Putative prolyl 4-hydroxylase; (1 of 1) 1.14.11.7 - Procollagen-proline 3-dioxygenase / Prolyl 3-hydroxylase | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATAATGAGCGTAGTTCCCACCAAGTTCTGGTCTCGTTTACTACCTGGCA |
Internal bar code: | GGTTGTCGACCACGTGCCAGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2258 |
LEAP-Seq percent confirming: | 95.2381 |
LEAP-Seq n confirming: | 60 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CATTGTTGTCGTGTTGCCGT |
Suggested primer 2: | TCTGCGGTTTGCTAGCTTGA |