Insertion junction: CLIP2.075765_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.075765
Chromosome:chromosome 13
Location:3011327
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g584200 GRL1 Glutaredoxin-like protein; (1 of 1) PF03595 - Voltage-dependent anion channel (SLAC1) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGACGTAGCTGAGTGGTCATCAGGCCACGCTGCAGAACGGGTCTCGGG
Internal bar code:TGAATTATGACTAAGGACCAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:555
LEAP-Seq percent confirming:20.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTAACACAGCGCTGCTATGC
Suggested primer 2:CGAGTTCCATTGCCAGCATG