Insertion junction: CLIP2.075778_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.075778
Chromosome:chromosome 10
Location:911086
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g424050 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCAGCTCAACTCTGGGGCGGCGAGCTGGAAGAAGTCCTCGACGGGAGC
Internal bar code:GAATACGCGTACGTCGCACAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:248
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGCGTCACCTTGCATACT
Suggested primer 2:GTTGCCCTTGCTGGTGAAAG