| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.075778 |
| Chromosome: | plastome |
| Location: | 60235 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802288 | rps7,2716973,ChreCp024 | 30S ribosomal protein S7; (1 of 2) K02992 - small subunit ribosomal protein S7 (RP-S7, MRPS7, rpsG) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACCGCCACTGCCTGCTTCCTCCTTCCCCTTCGGGCAAGTAAACTTCGG |
| Internal bar code: | GTGCGCTTTTGTAGCAGATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 136 |
| LEAP-Seq percent confirming: | 89.4737 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCCATTGCTAACGCCATG |
| Suggested primer 2: | AGTCTCGCGAAGAGCCTAGA |