| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075786 |
| Chromosome: | chromosome 5 |
| Location: | 2092349 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236802 | (1 of 71) IPR016181 - Acyl-CoA N-acyltransferase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATATGCTGGAGTGCATGGCCACGCACTGCCGCTTAGGCCACCGCCCAA |
| Internal bar code: | CGGTATCCAGTAATTTAGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1375 |
| LEAP-Seq percent confirming: | 70.4225 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCGGAACTTGATTAGCAG |
| Suggested primer 2: | ACCGCATGCAGGTAGGTATG |