| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.075794 |
| Chromosome: | chromosome 12 |
| Location: | 5181084 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527000 | CDPK14 | (1 of 1) PF07714//PF13202//PF13833 - Protein tyrosine kinase (Pkinase_Tyr) // EF hand (EF-hand_5) // EF-hand domain pair (EF-hand_8); Calcium/calmodulin-dependent protein kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCTAACCTCCGTGGCCTAGCCATACAAGTAATAGTCCAGCAGTCCTAC |
| Internal bar code: | AACTTGTGCAAACTAAAGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5220 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCTTGCCCTTGGACTTAT |
| Suggested primer 2: | AAATGGTCAGAGGTCAGGCG |