| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.075794 |
| Chromosome: | chromosome 12 |
| Location: | 5181092 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527000 | CDPK14 | (1 of 1) PF07714//PF13202//PF13833 - Protein tyrosine kinase (Pkinase_Tyr) // EF hand (EF-hand_5) // EF-hand domain pair (EF-hand_8); Calcium/calmodulin-dependent protein kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTAGAAGCTCCCAGGCCTTGGGGTGGGCGCGGGATGACAGGACGCGAGG |
| Internal bar code: | AACTTGTGCAAACTAAAGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 342 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATGGTCAGAGGTCAGGCG |
| Suggested primer 2: | GCCCTTGCCCTTGGACTTAT |