| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.075802 |
| Chromosome: | chromosome 8 |
| Location: | 2863945 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374100 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGACCTGGCCCGTTTGCTTTTACACAGACGGAGGCCAACAGTGTTAGT |
| Internal bar code: | CTTCCCTAAGATTGGAACGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 891 |
| LEAP-Seq percent confirming: | 63.0435 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTGTAAACCGCGTCGTGA |
| Suggested primer 2: | CGCCGTTACTAGGGGAATCC |