Insertion junction: CLIP2.075820_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.075820
Chromosome:chromosome 8
Location:4389792
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre08.g385050 MAPK2 Mitogen-Activated Protein Kinase Homolog 2; (1 of 3) K04371 - mitogen-activated protein kinase 1/3 (MAPK1_3) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAACGCGAAGTCGAGGCGCTCTCGATAGCTGTGGAGCGCGCCAACAGT
Internal bar code:CCTCAGAGGGATACAGTGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:881
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:CGGCCATAGGTGAGCAGAAA
Suggested primer 2:CCTTGCCGATGACCTCCTTT