| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075834 |
| Chromosome: | chromosome 10 |
| Location: | 271695 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419500 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGCTGGACTCGAAAGCGCAAGTTGCACAAATAGGCGCACAAATTGGA |
| Internal bar code: | TTGTTTTATGAAGTTCCAGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1127 |
| LEAP-Seq percent confirming: | 42.5 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGCTACCTGTGGCTATC |
| Suggested primer 2: | GCGTAGTCAAATGCGACGTC |