Insertion junction: CLIP2.075835_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.075835
Chromosome:plastome
Location:10640
Confidence (%):40
Locus systematic id Locus common name Defline Feature
CreCp.g802266 2716978,psbK,ChreCp004 (1 of 1) K02712 - photosystem II PsbK protein (psbK); photosystem II K protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTGTATTCTTTATTTTATTAGCTTTTGTTTGGCAAGCAGCTGTTAGTT
Internal bar code:TATAGATAGCAGCTCTTGCACA

Confirmation - LEAP-Seq

LEAP-Seq distance:335
LEAP-Seq percent confirming:80.9524
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTCAAACTTAGCACGTGACA
Suggested primer 2:CATAGGGTCGCAGGTTCGAA