| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.075840 |
| Chromosome: | chromosome 3 |
| Location: | 4298170 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g800329 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGACTCTGACTCTGGTTCGCAATTACGTCAGTGTTAGCTGGATTGTTG |
| Internal bar code: | CTATGGGTTCGCCATGCGACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2066 |
| LEAP-Seq percent confirming: | 68.1818 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGGGGTGCTTCATCTGTG |
| Suggested primer 2: | ACATCCACGTCGAATGGCTT |