Insertion junction: CLIP2.075844_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.075844
Chromosome:chromosome 16
Location:2907019
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g663350 CLPX1,CLPX ClpX chaperone; (1 of 1) K03544 - ATP-dependent Clp protease ATP-binding subunit ClpX (clpX, CLPX) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGTTATGAAGGGGGCAAACTGCGCCGCGAATGTAAATAAGATGTATGTG
Internal bar code:AGAATGATCTATATAAGGTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:3844
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CGTATCCTCTAACGGCCACC
Suggested primer 2:TGCTTGGTCGCTCATGCTTA