Insertion junction: CLIP2.075866_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.075866
Chromosome:chromosome 5
Location:860995
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre05.g246200 (1 of 2) PTHR24347//PTHR24347:SF159 - SERINE/THREONINE-PROTEIN KINASE // SERINE/THREONINE-PROTEIN KINASE STK11 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGAAACATCTACGCCGATCATTTGCGGCCTCGACTTGCGCCATTTGGAG
Internal bar code:TAGGAAGGCGCCTCAAGAAAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:109
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACGCTTGCAATCCAACCAA
Suggested primer 2:CTCTGCCCCTTCTGTTACCG