Insertion junction: CLIP2.075883_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.075883
Chromosome:chromosome 17
Location:1973073
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre17.g710300 PHC31 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 31 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGCTTGACGGAGCCGCGGCAGGCGTCCTTGGTCCACCACTCAATCTTG
Internal bar code:CTGTTAGCTTAAGGTTGTGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:222
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTGGGTTACGAGGACTGC
Suggested primer 2:CAAACACTCCCAACAGCTGC