| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.075892 |
| Chromosome: | chromosome 10 |
| Location: | 3632709 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g801148 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGATAATACGCCACGCCAGCGCCTGGAAAGAAACGCCGCCCAAAAGGA |
| Internal bar code: | CGATCGGATACTCCCAGGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1252 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACCGGATGCAACATGCT |
| Suggested primer 2: | TTGCGTTGGTTGTCAGCATG |