Insertion junction: CLIP2.075893_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.075893
Chromosome:chromosome 14
Location:2432985
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g623650 ADH7 Alcohol dehydrogenase; (1 of 3) 1.1.1.2 - Alcohol dehydrogenase (NADP(+)) / Aldehyde reductase (NADPH) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTTTTATCATTCAGGCCCACATGGTGCCCGCGTGCGATCGCGCTGTTAA
Internal bar code:ACGCTGTAACGACAGTATTATA

Confirmation - LEAP-Seq

LEAP-Seq distance:3595
LEAP-Seq percent confirming:95.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTAAAGCCCTTAATGCGC
Suggested primer 2:GCCGAGTAAACCAAGACCCA