Insertion junction: CLIP2.075908_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.075908
Chromosome:chromosome 16
Location:7021288
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g673729 CPN11 Chaperonin 11, chloroplastic; (1 of 1) PTHR10772:SF13 - CHLOROPLAST CHAPERONIN 10 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGGAACGCGCGGCACAGTTTGAGTGTTGAGGGGGGGGAAGACAAGGAA
Internal bar code:CGATCATGCGCTAGTGGTTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3758
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCCCGTCGAGTAGCTAAG
Suggested primer 2:GTTGCTGGCGGATATTGCTG