Insertion junction: CLIP2.075911_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.075911
Chromosome:chromosome 6
Location:4002130
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278209 PRL8 Predicted extracellular protein; (1 of 4) PTHR10334//PTHR10334:SF224 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED // SUBFAMILY NOT NAMED 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGGACATCCACAGGAAAGCCGCTGGAAGGCCGCAGCGGACGCATTTCA
Internal bar code:TCGTTTAAACTTTATATTACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2001
LEAP-Seq percent confirming:98.4848
LEAP-Seq n confirming:65
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:66

Suggested primers for confirmation by PCR

Suggested primer 1:CGAAACAACGCTGCGATAGG
Suggested primer 2:CCGGACATTTCGGGATCGAT