| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.075914 |
| Chromosome: | chromosome 4 |
| Location: | 1339831 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214500 | IDH3 | (1 of 1) 1.1.1.42 - Isocitrate dehydrogenase (NADP(+)) / oxalosuccinate carboxylase; Isocitrate dehydrogenase, NADP-dependent | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTAATGGGGGTGCCTGGCGACGGAGGATAAGGGATGCCGGATGGTTGG |
| Internal bar code: | ATCAGTATGGCAACCTATGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 92 |
| LEAP-Seq percent confirming: | 14.2857 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCAGCTCCTCTTCCTCCT |
| Suggested primer 2: | TGAGGGAGGACTGAGAAGGG |