| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075925 |
| Chromosome: | chromosome 2 |
| Location: | 4716048 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108200 | CGL80 | Conserved in the Green Lineage; (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
| Cre02.g108250 | (1 of 2) PF02696 - Uncharacterized ACR, YdiU/UPF0061 family (UPF0061) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGCATGGGCATACCACATTGTTGAACGTCCGCCTCACATGCCGGCCG |
| Internal bar code: | TTCTTTCGGGAACGGGGGTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1964 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTGTGTTGCGTGTACAC |
| Suggested primer 2: | TACCGGCTGCAACTAACCAG |