| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.075937 |
| Chromosome: | chromosome 14 |
| Location: | 1910041 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g801622 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTACCCGCCGGGGAGGGCCTCGTGATTCCGGACTGCGCAACCATGGCG |
| Internal bar code: | TTGGTCAGTGTTGATCGATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2035 |
| LEAP-Seq percent confirming: | 63.6364 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAACAAACCGCAATCCA |
| Suggested primer 2: | CTGATACCGTACCGTGCTCC |