| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075938 |
| Chromosome: | chromosome 13 |
| Location: | 4056470 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g590850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATAGTGCCTCTTGAGCAACGTCGTCCCCAGAACTAAAAAATGACCTTGA |
| Internal bar code: | CGGCCAGACGACAACATGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2747 |
| LEAP-Seq percent confirming: | 53.1915 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTTGTACTCGGGCTGTA |
| Suggested primer 2: | ATGCCGAGTCTCCAAACAGG |