| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075940 |
| Chromosome: | chromosome 2 |
| Location: | 1714508 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g086000 | ARS8 | Arylsulfatase; (1 of 1) IPR002591//IPR003882//IPR017850 - Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase // Pistil-specific extensin-like protein // Alkaline-phosphatase-like, core domain | 3'UTR |
| Cre02.g086050 | PUS4 | (1 of 1) 4.2.1.70//5.4.99.12//5.4.99.45 - Pseudouridylate synthase / Uracil hydrolyase // tRNA pseudouridine(38-40) synthase / tRNA pseudouridylate synthase I // tRNA pseudouridine(38/39) synthase / Pseudouridine synthase 3; RNA pseudouridine synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCCTACGGTGTATTGGGGCCATCCGGTGAAAGGCCGCGGTTTTGCGA |
| Internal bar code: | GTTAAGGCCCTAGCCGTCTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1931 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 95 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 95 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCATGCCACACCCATACCC |
| Suggested primer 2: | TGTGCACCGTGAGTAGTGTC |