Insertion junction: CLIP2.075946_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.075946
Chromosome:chromosome 3
Location:1266757
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g149900 (1 of 1) IPR027369 - DinB/YfiT-like putative metal-dependent hydrolase 3'UTR
Cre03.g149950 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGTTTGTGCGCGCATTCGATGCGGGCCGCTGGGCAGTGGACTGCTGCT
Internal bar code:GTGTGGCAACGAAGTGGAGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1177
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:TAGGTGTGATACGGCCTGGA
Suggested primer 2:CTCTCTCTGCCTCTCCTGGT